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KMID : 0545120000100010069
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Journal of Microbiology and Biotechnology
2000 Volume.10 No. 1 p.69 ~ p.74
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Optimization of Culture Conditions for Erythritol Production by Torula sp.
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Kim, Kyung Ah
Noh, Bong Soo/Lee, Jung Kul/Kim, Sang Yong/Park, Yong Cheol/Oh, Deok Kun
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Abstract
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The medium for erythritol production by Torula sp. in a 500-㎖ baffled flask was optimized to be 300g/1 sucrose, 10g/1 yeast extract, 3g/l KH_2PO_4, and 10㎎/l CuSO_4·5H_2O at 34℃ with initial pH of 5.5. Using this optimal medium, erythritol of 166g/l was obtained after 140h of cultivation, corresponding to 55.3% of the erythritol yield from sucrose with a productivity of 1.11g/l/h. Optimal concentrations of carbon and nitrogen sources in a fermentor were higher than that in a flask due to the higher oxygen supply of the fermentor. Employing the medium containing 300g/l or 400g/l sucrose for the determination of optimal C/N ratio, the C/N ratio was found to be more important than the nitrogen concentration for effective erythritol production. The optimal ratio of yeast extract to sucrose (g/g) was 20. The yield and productivity of erythritol were maximal in the medium containing 400g/l sucrose and 20g/l yeast extract. When dissolved oxygen in the culture was increased, the cell mass increased but the erythritol production was maximal in the range of 5 to 10% of dissolved oxygen. Under the optimal culture condition of the fermentor, a final erythritol concentration of 200g/l was obtained after 120h with a yield of 50% and the productivity was 1.67g/l/h. The yield was the highest among erythritol-producing microorganisms.
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